Fast, Simple and Accurate
Nucleic Acid Quantification
For your
Helixyte™ Green Fluorimetric DNA Quantitation Kits for use with fluorescence microplate readers
For your
The Colibri+ takes microvolume quantification of DNA, RNA and protein samples to a whole new level
Accurate nucleic acid quantitation is an essential step in DNA and RNA sample preparation and regularly contributes to the success (or failure) of various downstream molecular experiments. Methods commonly used to quantify DNA and RNA include absorbance (UV-Vis) measurements, fluorescence measurements, and real-time polymerase chain reaction (qPCR). While each method presents its advantages, choosing the right technology can save significant time and money and depends mainly on the experimental need and the intended downstream application.
For efficient and effective nucleic acid quantitation, AAT Bioquest offers a range of products to help you get the job done , including labelling solutions for electrophoresis gels, Plate readers and tube fluorimeters.
Gelite™ Safe DNA Gel stain. Nucleic acid stains for agarose and polyacrylamide gel electrophoresis.
Figure 1. Comparison of DNA detection in 1% agarose gel in TBE buffer using Gelite™ Safe, EtBr, and SYBR® Safe. Two-fold serial dilutions of 1 kb DNA ladder were loaded in amounts of 100 ng, 50 ng, and 25 ng from left to right. Gels were stained for 60 minutes with Gelite™ Safe, EtBr, and SYBR® Safe according to the manufacturer's recommended concentrations and imaged using the ChemiDoc™ Imaging System (Bio-Rad®). Gels were illuminated using a 300 nm transilluminator fitted with a GelGreen filter.
Figure 2. Comparison of DNA detection in 1% agarose gel in TBE buffer using Gelite™ Safe, SYBR® Safe, and EtBr. Two-fold serial dilutions of 1 kb DNA ladder were loaded in amounts of 86 ng, 43 ng, 21.5 ng, 10.7 ng, 5.3 ng, 2.6 ng, 1.3 ng, and 0.5 ng from left to right. Gels were imaged using a 300 nm transilluminator in ChemiDoc™ Imaging System (Bio-Rad®).
Gelite™ Safe DNA Gel Stain. Gelite™ Safe exhibits a considerable fluorescence enhancement by several orders of magnitude greater than that of EtBr. Gelite™ Safe was optimized to be compatible with various instruments, including UV and blue-light transiluminators, gel documentation systems, and laser scanners. It is the first single formulation that can be used in either the green or red channel at your preference. Unlike the membrane-permeant SYBR® Green, which is highly toxic to cells and the environment, the membrane-impermeant properties of Gelite™ Safe make it a much safer and noncytotoxic alternative.
Transilluminator
Excitation: 254 nm or 300 nm
Emission: Long path green filter (ex. SYBR or GelStar)
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Product ▲ ▼ |
Ex (nm)¹ ▲ ▼ |
Filter² ▲ ▼ |
Unit Size ▲ ▼ |
Cat No. ▲ ▼ |
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Gelite™ Green Nucleic Acid Gel Staining Kit |
254 nm or 300 nm |
Long path green filter |
1 Kit |
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Gelite™ Orange Nucleic Acid Gel Staining Kit |
254 nm or 300 nm |
Long path green filter |
1 Kit |
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Gelite™ Safe DNA Gel Stain *10,000X DMSO Solution* |
254 nm, 300 nm or 520 nm |
Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters |
100 µL |
|
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Gelite™ Safe DNA Gel Stain *10,000X DMSO Solution* |
254 nm, 300 nm or 520 nm |
Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters |
500 µL |
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Gelite™ Safe DNA Gel Stain *10,000X DMSO Solution* |
254 nm, 300 nm or 520 nm |
Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters |
1 mL |
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Gelite™ Safe DNA Gel Stain *10,000X DMSO Solution* |
254 nm, 300 nm or 520 nm |
Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters |
10 mL |
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Gelite™ Safe DNA Gel Stain *10,000X Water Solution* |
254 nm, 300 nm or 520 nm |
Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters |
100 µL |
|
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Gelite™ Safe DNA Gel Stain *10,000X Water Solution* |
254 nm, 300 nm or 520 nm |
Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters |
500 µL |
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Gelite™ Safe DNA Gel Stain *10,000X Water Solution* |
254 nm, 300 nm or 520 nm |
Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters |
1 mL |
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Gelite™ Safe DNA Gel Stain *10,000X Water Solution* |
254 nm, 300 nm or 520 nm |
Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters |
10 mL |
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Helixyte™ Gold Nucleic Acid Gel Stain *10,000X DMSO Solution* |
254 mn |
Long path green filter |
1 mL |
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Helixyte™ Green Nucleic Acid Gel Stain *10,000X DMSO Solution* |
254 mn |
Long path green filter |
1 mL |
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Helixyte™ Green Nucleic Acid Gel Stain *10,000X DMSO Solution* |
254 mn |
Long path green filter |
100 µL |
Helixyte™ Green Fluorimetric DNA Quantitation Kits for use with fluorescence microplate readers
Figure 1. Comparison of dsDNA dose response using the Helixyte Green™ (blue ) with InvitrogenTM Quant-iT™ PicoGreen® dsDNA Reagent (red ). dsNDA standards were incubated in cuvettes and measured using varian cary eclipse fluorescence spectrophotometer.
Tristar Multimode Plate Readers
Flexibility and sensitivity whenever you need it
Introducing the NEW Tristar family of modular multimode plate readers from Berthold, Germany. Configured ready for fluorescence and UV/vis nucleic quantification. With life time application support and flexible configuration, for when you need a new assay setup.
Helixyte™ Green is an excellent nucleic acid sensor that exhibits large fluorescence enhancement upon binding to dsDNA. It has the same spectral properties to those of PicoGreen®, thus a great replacement to PicoGreen® (PicoGreen® is the trademark of Invitrogen).
Fluorescence microplate reader
Excitation: 490 nm
Emission: 525 nm
Cutoff: 515 nm
Recommended plate: Solid black
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Product ▲ ▼ |
Ex/Em (nm) ▲ ▼ |
Target ▲ ▼ |
Detection Range ▲ ▼ |
Unit Size ▲ ▼ |
Cat No. ▲ ▼ |
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Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *High Sensitivity* |
490/525 |
dsDNA |
0.2-100 ng |
200 tests |
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Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *Optimized for Broad Dynamic Range* |
490/530 |
dsDNA |
2-1000 ng |
200 tests |
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Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *Optimized for Broad Dynamic Range* |
490/530 |
dsDNA |
2-1000 ng |
1000 tests |
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Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *Optimized for Microplate Readers* |
490/525 |
dsDNA |
0.2-100 ng |
200 tests |
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Helixyte™ Green Fluorimetric ssDNA Quantitation Kit *Optimized for Microplate Readers* |
490/525 |
ssDNA |
1-200 ng |
200 tests |
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Helixyte™ Green Fluorimetric Total Nucleic Acid Quantitation Kit *Optimized for Microplate Readers* |
490/525 |
dsDNA, ssDNA, RNA, oligos |
as little as 0.3 ng |
200 tests |
Accurate DNA, RNA & Protein Quantification in less than 3 seconds
Colibri LB 915 Microvolume Spectrophotometer
The Colibri+ takes microvolume quantification of DNA, RNA and protein samples to a whole new level. The system has been designed to help you accelerate your research combining ultra-fast measurement in less than 3 seconds with intuitive 7-inch touchscreen stand-alone operation.
Get confidence in the quality of your sample with reliable UV/VIS spectroscopy from as low as 1 µL of sample.
Fast measurement
Read your samples in less than 3 seconds.
Reliable results
CV of absorbance < 1%.
Broad detection range
2 – 20.000 ng/µL for dsDNA.
0.06 – 600 mg/mL for BSA.
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