Fast, Simple and Accurate
Nucleic Acid Quantification
For your
Helixyte™ Green Fluorimetric DNA Quantitation Kits for use with fluorescence microplate readers
For your
Portelite™ Fluorimetric Nucleic Acid Quantitation Kits for use with the CytoCite™ and Qubit® fluorometers
Accurate nucleic acid quantitation is an essential step in DNA and RNA sample preparation and regularly contributes to the success (or failure) of various downstream molecular experiments. Methods commonly used to quantify DNA and RNA include absorbance (UV-Vis) measurements, fluorescence measurements, and real-time polymerase chain reaction (qPCR). While each method presents its advantages, choosing the right technology can save significant time and money and depends mainly on the experimental need and the intended downstream application.
For efficient and effective nucleic acid quantitation, AAT Bioquest offers a range of products to help you get the job done , including labelling solutions for electrophoresis gels, Plate readers and tube fluorimeters.
Gelite™ Safe DNA Gel stain. Nucleic acid stains for agarose and polyacrylamide gel electrophoresis.
Figure 1. Comparison of DNA detection in 1% agarose gel in TBE buffer using Gelite™ Safe, EtBr, and SYBR® Safe. Two-fold serial dilutions of 1 kb DNA ladder were loaded in amounts of 100 ng, 50 ng, and 25 ng from left to right. Gels were stained for 60 minutes with Gelite™ Safe, EtBr, and SYBR® Safe according to the manufacturer's recommended concentrations and imaged using the ChemiDoc™ Imaging System (Bio-Rad®). Gels were illuminated using a 300 nm transilluminator fitted with a GelGreen filter.
Figure 2. Comparison of DNA detection in 1% agarose gel in TBE buffer using Gelite™ Safe, SYBR® Safe, and EtBr. Two-fold serial dilutions of 1 kb DNA ladder were loaded in amounts of 86 ng, 43 ng, 21.5 ng, 10.7 ng, 5.3 ng, 2.6 ng, 1.3 ng, and 0.5 ng from left to right. Gels were imaged using a 300 nm transilluminator in ChemiDoc™ Imaging System (Bio-Rad®).
Gelite™ Safe DNA Gel Stain. Gelite™ Safe exhibits a considerable fluorescence enhancement by several orders of magnitude greater than that of EtBr. Gelite™ Safe was optimized to be compatible with various instruments, including UV and blue-light transiluminators, gel documentation systems, and laser scanners. It is the first single formulation that can be used in either the green or red channel at your preference. Unlike the membrane-permeant SYBR® Green, which is highly toxic to cells and the environment, the membrane-impermeant properties of Gelite™ Safe make it a much safer and noncytotoxic alternative.
Transilluminator
Excitation: 254 nm or 300 nm
Emission: Long path green filter (ex. SYBR or GelStar)
Product ▲ ▼ |
Ex (nm)¹ ▲ ▼ |
Filter² ▲ ▼ |
Unit Size ▲ ▼ |
Cat No. ▲ ▼ |
Gelite™ Green Nucleic Acid Gel Staining Kit |
254 nm or 300 nm |
Long path green filter |
1 Kit |
|
Gelite™ Orange Nucleic Acid Gel Staining Kit |
254 nm or 300 nm |
Long path green filter |
1 Kit |
|
Gelite™ Safe DNA Gel Stain *10,000X DMSO Solution* |
254 nm, 300 nm or 520 nm |
Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters |
100 µL |
|
Gelite™ Safe DNA Gel Stain *10,000X DMSO Solution* |
254 nm, 300 nm or 520 nm |
Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters |
500 µL |
|
Gelite™ Safe DNA Gel Stain *10,000X DMSO Solution* |
254 nm, 300 nm or 520 nm |
Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters |
1 mL |
|
Gelite™ Safe DNA Gel Stain *10,000X DMSO Solution* |
254 nm, 300 nm or 520 nm |
Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters |
10 mL |
|
Gelite™ Safe DNA Gel Stain *10,000X Water Solution* |
254 nm, 300 nm or 520 nm |
Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters |
100 µL |
|
Gelite™ Safe DNA Gel Stain *10,000X Water Solution* |
254 nm, 300 nm or 520 nm |
Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters |
500 µL |
|
Gelite™ Safe DNA Gel Stain *10,000X Water Solution* |
254 nm, 300 nm or 520 nm |
Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters |
1 mL |
|
Gelite™ Safe DNA Gel Stain *10,000X Water Solution* |
254 nm, 300 nm or 520 nm |
Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters |
10 mL |
|
Helixyte™ Gold Nucleic Acid Gel Stain *10,000X DMSO Solution* |
254 mn |
Long path green filter |
1 mL |
|
Helixyte™ Green Nucleic Acid Gel Stain *10,000X DMSO Solution* |
254 mn |
Long path green filter |
1 mL |
|
Helixyte™ Green Nucleic Acid Gel Stain *10,000X DMSO Solution* |
254 mn |
Long path green filter |
100 µL |
Helixyte™ Green Fluorimetric DNA Quantitation Kits for use with fluorescence microplate readers
Figure 1. Comparison of dsDNA dose response using the Helixyte Green™ (blue ) with InvitrogenTM Quant-iT™ PicoGreen® dsDNA Reagent (red ). dsNDA standards were incubated in cuvettes and measured using varian cary eclipse fluorescence spectrophotometer.
Helixyte™ Green is an excellent nucleic acid sensor that exhibits large fluorescence enhancement upon binding to dsDNA. It has the same spectral properties to those of PicoGreen®, thus a great replacement to PicoGreen® (PicoGreen® is the trademark of Invitrogen).
Fluorescence microplate reader
Excitation: 490 nm
Emission: 525 nm
Cutoff: 515 nm
Recommended plate: Solid black
Product ▲ ▼ |
Ex/Em (nm) ▲ ▼ |
Target ▲ ▼ |
Detection Range ▲ ▼ |
Unit Size ▲ ▼ |
Cat No. ▲ ▼ |
Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *High Sensitivity* |
490/525 |
dsDNA |
0.2-100 ng |
200 tests |
|
Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *Optimized for Broad Dynamic Range* |
490/530 |
dsDNA |
2-1000 ng |
200 tests |
|
Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *Optimized for Broad Dynamic Range* |
490/530 |
dsDNA |
2-1000 ng |
1000 tests |
|
Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *Optimized for Microplate Readers* |
490/525 |
dsDNA |
0.2-100 ng |
200 tests |
|
Helixyte™ Green Fluorimetric ssDNA Quantitation Kit *Optimized for Microplate Readers* |
490/525 |
ssDNA |
1-200 ng |
200 tests |
|
Helixyte™ Green Fluorimetric Total Nucleic Acid Quantitation Kit *Optimized for Microplate Readers* |
490/525 |
dsDNA, ssDNA, RNA, oligos |
as little as 0.3 ng |
200 tests |
Tristar Multimode Plate Readers
Flexibility and sensitivity whenever you need it
Introducing the NEW Tristar family of modular multimode plate readers from Berthold, Germany. Configured ready for fluorescence and UV/vis nucleic quantification. With life time application support and flexible configuration, for when you need a new assay setup.
Portelite™ Fluorimetric Nucleic Acid Quantitation Kits for use with the CytoCite™ and Qubit® fluorometers
DNA Quantitation is a very important task in DNA sample preparations for various analyses. Portelite™ Fluorimetric DNA Quantitation Kit provides a rapid method to quantify dsDNA with Helixyte™ Green BR using a hand-held fluorometer. It is optimized for Cytocite™ and Qubit™ fluorometers. Portelite™ Fluorimetric DNA Quantitation assay is linear over three orders of magnitude. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is optimized for measuring dsDNA concentrations from 10 pg/µL to 10 ng/µL. Helixyte™ Green BR exhibits large fluorescence enhancement upon binding to dsDNA, and it is a few magnitudes more sensitive than UV absorbance readings.
Qubit Fluorometer
Excitation: 480 nm
Emission: 520 nm
Instrument specification(s): 0.2 mL, thin-wall PCR tube
CytoCite Fluorometer
Excitation: 480 nm
Emission: 520 nm
Instrument specification(s): 0.2 mL, thin-wall PCR tube
Product ▲ ▼ |
Platform ▲ ▼ |
Ex/Em (nm) ▲ ▼ |
Target ▲ ▼ |
Detection Range ▲ ▼ |
Unit Size ▲ ▼ |
Cat No. ▲ ▼ |
Portelite™ Fluorimetric DNA Quantitation Kit with Broad Dynamic Range |
CytoCite™ BG100 or Qubit® Fluorometer |
480/530 |
dsDNA |
2-1000 ng |
100 tests |
|
Portelite™ Fluorimetric High Sensitivity DNA Quantitation Kit |
CytoCite™ BG100 or Qubit® Fluorometer |
480/520 |
dsDNA |
0.2-100 ng |
100 tests |
|
Portelite™ Fluorimetric High Sensitivity DNA Quantitation Kit |
CytoCite™ BG100 or Qubit® Fluorometer |
480/520 |
dsDNA |
0.2-100 ng |
500 tests |
|
Portelite™ Fluorimetric RNA Quantitation Kit |
CytoCite™ BG100 or Qubit® Fluorometer |
480/530 |
RNA |
5-100 ng |
100 tests |
|
Portelite™ Fluorimetric RNA Quantitation Kit |
CytoCite™ BG100 or Qubit® Fluorometer |
480/530 |
RNA |
5-100 ng |
500 tests |
|
Portelite™ Fluorimetric ssDNA Quantitation Kit |
CytoCite™ BG100 or Qubit® Fluorometer |
480/530 |
ssDNA |
1-200 ng |
200 tests |
|
Portelite™ Fluorimetric Total Nucleic Acid Quantitation Kit |
CytoCite™ BG100 or Qubit® Fluorometer |
480/530 |
dsDNA, ssDNA, RNA, oligos |
as little as 0.3 ng |
200 tests |
Portelite™ runs on all Qubit tube fluorimeters
Please contact our friendly team to assist with product selection, pricing and delivery information. Freephone 0800 724 633 or email info@scienze.nz
CytoCite™ Fluorometer
Fast, Specific & Accurate Quantitation
Fast, Specific & Accurate Quantitation in a small benchtop form fluorimeter, sample volume's down to 150uL...
Need more information / Questions?
Need more information / Questions?
Please contact us by phone or email:
Freephone 0800 724 633
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