Fast, Simple and Accurate

Nucleic Acid Quantification

For your

Gelite™ & Helixyte™ Nucleic acid stains for agarose and polyacrylamide gel electrophoresis

For your

Microplate Readers ›

Helixyte™ Green Fluorimetric DNA Quantitation Kits for use with fluorescence microplate readers

For your

Tube Fluorometers ›

Portelite™ Fluorimetric Nucleic Acid Quantitation Kits for use with the CytoCite™ and Qubit® fluorometers

Accurate nucleic acid quantitation is an essential step in DNA and RNA sample preparation and regularly contributes to the success (or failure) of various downstream molecular experiments. Methods commonly used to quantify DNA and RNA include absorbance (UV-Vis) measurements, fluorescence measurements, and real-time polymerase chain reaction (qPCR). While each method presents its advantages, choosing the right technology can save significant time and money and depends mainly on the experimental need and the intended downstream application.

For efficient and effective nucleic acid quantitation, AAT Bioquest offers a range of products to help you get the job done , including labelling solutions for electrophoresis gels, Plate readers and tube fluorimeters.

Gelite™ Safe DNA Gel stain. Nucleic acid stains for agarose and polyacrylamide gel electrophoresis.

Figure 1. Comparison of DNA detection in 1% agarose gel in TBE buffer using Gelite™ Safe, EtBr, and SYBR® Safe. Two-fold serial dilutions of 1 kb DNA ladder were loaded in amounts of 100 ng, 50 ng, and 25 ng from left to right. Gels were stained for 60 minutes with Gelite™ Safe, EtBr, and SYBR® Safe according to the manufacturer's recommended concentrations and imaged using the ChemiDoc™ Imaging System (Bio-Rad®). Gels were illuminated using a 300 nm transilluminator fitted with a GelGreen filter.

Figure 2. Comparison of DNA detection in 1% agarose gel in TBE buffer using Gelite™ Safe, SYBR® Safe, and EtBr. Two-fold serial dilutions of 1 kb DNA ladder were loaded in amounts of 86 ng, 43 ng, 21.5 ng, 10.7 ng, 5.3 ng, 2.6 ng, 1.3 ng, and 0.5 ng from left to right. Gels were imaged using a 300 nm transilluminator in ChemiDoc™ Imaging System (Bio-Rad®).

Gelite™ Safe DNA Gel Stain. Gelite™ Safe exhibits a considerable fluorescence enhancement by several orders of magnitude greater than that of EtBr. Gelite™ Safe was optimized to be compatible with various instruments, including UV and blue-light transiluminators, gel documentation systems, and laser scanners. It is the first single formulation that can be used in either the green or red channel at your preference. Unlike the membrane-permeant SYBR® Green, which is highly toxic to cells and the environment, the membrane-impermeant properties of Gelite™ Safe make it a much safer and noncytotoxic alternative.

Transilluminator

Excitation: 254 nm or 300 nm

Emission: Long path green filter (ex. SYBR or GelStar)

Product ▲ ▼	Ex (nm)¹ ▲ ▼	Filter² ▲ ▼	Unit Size ▲ ▼	Cat No. ▲ ▼
Gelite™ Green Nucleic Acid Gel Staining Kit	254 nm or 300 nm	Long path green filter	1 Kit	17589
Gelite™ Orange Nucleic Acid Gel Staining Kit	254 nm or 300 nm	Long path green filter	1 Kit	17594
Gelite™ Safe DNA Gel Stain 10,000X DMSO Solution	254 nm, 300 nm or 520 nm	Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters	100 µL	17704
Gelite™ Safe DNA Gel Stain 10,000X DMSO Solution	254 nm, 300 nm or 520 nm	Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters	500 µL	17705
Gelite™ Safe DNA Gel Stain 10,000X DMSO Solution	254 nm, 300 nm or 520 nm	Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters	1 mL	17706
Gelite™ Safe DNA Gel Stain 10,000X DMSO Solution	254 nm, 300 nm or 520 nm	Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters	10 mL	17707
Gelite™ Safe DNA Gel Stain 10,000X Water Solution	254 nm, 300 nm or 520 nm	Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters	100 µL	17700
Gelite™ Safe DNA Gel Stain 10,000X Water Solution	254 nm, 300 nm or 520 nm	Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters	500 µL	17701
Gelite™ Safe DNA Gel Stain 10,000X Water Solution	254 nm, 300 nm or 520 nm	Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters	1 mL	17702
Gelite™ Safe DNA Gel Stain 10,000X Water Solution	254 nm, 300 nm or 520 nm	Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters	10 mL	17703
Helixyte™ Gold Nucleic Acid Gel Stain 10,000X DMSO Solution	254 mn	Long path green filter	1 mL	17595
Helixyte™ Green Nucleic Acid Gel Stain 10,000X DMSO Solution	254 mn	Long path green filter	1 mL	17590
Helixyte™ Green Nucleic Acid Gel Stain 10,000X DMSO Solution	254 mn	Long path green filter	100 µL	1760

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Helixyte™ Green Fluorimetric DNA Quantitation Kits for use with fluorescence microplate readers

figure-for-helixyte-green-fluorimetric-dsdna-quantitation-kit-optimized-for-microplate-readers_ekdvl

Figure 1. Comparison of dsDNA dose response using the Helixyte Green™ (blue ) with Invitrogen^TM Quant-iT™ PicoGreen® dsDNA Reagent (red ). dsNDA standards were incubated in cuvettes and measured using varian cary eclipse fluorescence spectrophotometer.

Helixyte™ Green is an excellent nucleic acid sensor that exhibits large fluorescence enhancement upon binding to dsDNA. It has the same spectral properties to those of PicoGreen®, thus a great replacement to PicoGreen® (PicoGreen® is the trademark of Invitrogen).

Fluorescence microplate reader

Excitation: 490 nm

Emission: 525 nm

Cutoff: 515 nm

Recommended plate: Solid black

Product ▲ ▼	Ex/Em (nm) ▲ ▼	Target ▲ ▼	Detection Range ▲ ▼	Unit Size ▲ ▼	Cat No. ▲ ▼
Helixyte™ Green Fluorimetric dsDNA Quantitation Kit High Sensitivity	490/525	dsDNA	0.2-100 ng	200 tests	17651
Helixyte™ Green Fluorimetric dsDNA Quantitation Kit Optimized for Broad Dynamic Range	490/530	dsDNA	2-1000 ng	200 tests	17645
Helixyte™ Green Fluorimetric dsDNA Quantitation Kit Optimized for Broad Dynamic Range	490/530	dsDNA	2-1000 ng	1000 tests	17646
Helixyte™ Green Fluorimetric dsDNA Quantitation Kit Optimized for Microplate Readers	490/525	dsDNA	0.2-100 ng	200 tests	17650
Helixyte™ Green Fluorimetric ssDNA Quantitation Kit Optimized for Microplate Readers	490/525	ssDNA	1-200 ng	200 tests	17623
Helixyte™ Green Fluorimetric Total Nucleic Acid Quantitation Kit Optimized for Microplate Readers	490/525	dsDNA, ssDNA, RNA, oligos	as little as 0.3 ng	200 tests	17630

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Tristar Multimode Plate Readers
Flexibility and sensitivity whenever you need it

Introducing the NEW Tristar family of modular multimode plate readers from Berthold, Germany. Configured ready for fluorescence and UV/vis nucleic quantification. With life time application support and flexible configuration, for when you need a new assay setup.

Introducing the NEW Tristar 3

Simplicity and sensitivity for all levels of experience

Learn more ›

Introducing the NEW Tristar 5

Flexibility and sensitivity whenever you need it

Learn more ›

Portelite™ Fluorimetric Nucleic Acid Quantitation Kits for use with the CytoCite™ and Qubit® fluorometers

graph-for-portelite-fluorimetric-dna-quantitation-kit-with-broad-dynamic-range-optimized-for-cytocite-and-qubit-fluorometers_R0zha

Figure 1. DNA standard curve generated using Portelite™ Fluorimetric DNA Quantitation Kit with Broad Dynamic Range. Fluorescence intensity was quantified using green fluorescence channel, regression model was calculated using linear fit.

DNA Quantitation is a very important task in DNA sample preparations for various analyses. Portelite™ Fluorimetric DNA Quantitation Kit provides a rapid method to quantify dsDNA with Helixyte™ Green BR using a hand-held fluorometer. It is optimized for Cytocite™ and Qubit™ fluorometers. Portelite™ Fluorimetric DNA Quantitation assay is linear over three orders of magnitude. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is optimized for measuring dsDNA concentrations from 10 pg/µL to 10 ng/µL. Helixyte™ Green BR exhibits large fluorescence enhancement upon binding to dsDNA, and it is a few magnitudes more sensitive than UV absorbance readings.

Qubit Fluorometer
Excitation: 480 nm
Emission: 520 nm
Instrument specification(s): 0.2 mL, thin-wall PCR tube

CytoCite Fluorometer
Excitation: 480 nm
Emission: 520 nm
Instrument specification(s): 0.2 mL, thin-wall PCR tube

Product ▲ ▼	Platform ▲ ▼	Ex/Em (nm) ▲ ▼	Target ▲ ▼	Detection Range ▲ ▼	Unit Size ▲ ▼	Cat No. ▲ ▼
Portelite™ Fluorimetric DNA Quantitation Kit with Broad Dynamic Range	CytoCite™ BG100 or Qubit® Fluorometer	480/530	dsDNA	2-1000 ng	100 tests	17665
Portelite™ Fluorimetric High Sensitivity DNA Quantitation Kit	CytoCite™ BG100 or Qubit® Fluorometer	480/520	dsDNA	0.2-100 ng	100 tests	17660
Portelite™ Fluorimetric High Sensitivity DNA Quantitation Kit	CytoCite™ BG100 or Qubit® Fluorometer	480/520	dsDNA	0.2-100 ng	500 tests	17661
Portelite™ Fluorimetric RNA Quantitation Kit	CytoCite™ BG100 or Qubit® Fluorometer	480/530	RNA	5-100 ng	100 tests	17658
Portelite™ Fluorimetric RNA Quantitation Kit	CytoCite™ BG100 or Qubit® Fluorometer	480/530	RNA	5-100 ng	500 tests	17659
Portelite™ Fluorimetric ssDNA Quantitation Kit	CytoCite™ BG100 or Qubit® Fluorometer	480/530	ssDNA	1-200 ng	200 tests	17625
Portelite™ Fluorimetric Total Nucleic Acid Quantitation Kit	CytoCite™ BG100 or Qubit® Fluorometer	480/530	dsDNA, ssDNA, RNA, oligos	as little as 0.3 ng	200 tests	17631

Portelite™ runs on all Qubit tube fluorimeters

Please contact our friendly team to assist with product selection, pricing and delivery information. Freephone 0800 724 633 or email info@scienze.nz

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