Fast, Simple and Accurate

Nucleic Acid Quantification

Gelite620

Gelite™ &  Helixyte™ Nucleic acid stains for agarose and polyacrylamide gel electrophoresis

Microplate620

Helixyte™ Green Fluorimetric DNA Quantitation Kits for use with fluorescence microplate readers

CytoCite620

Portelite™ Fluorimetric Nucleic Acid Quantitation Kits for use with the CytoCite™ and Qubit® fluorometers

Accurate nucleic acid quantitation is an essential step in DNA and RNA sample preparation and regularly contributes to the success (or failure) of various downstream molecular experiments. Methods commonly used to quantify DNA and RNA include absorbance (UV-Vis) measurements, fluorescence measurements, and real-time polymerase chain reaction (qPCR). While each method presents its advantages, choosing the right technology can save significant time and money and depends mainly on the experimental need and the intended downstream application.

For efficient and effective nucleic acid quantitation, AAT Bioquest offers a range of products to help you get the job done , including labelling solutions for electrophoresis gels, Plate readers and tube fluorimeters.

Gelite™ Safe DNA Gel stain. Nucleic acid stains for agarose and polyacrylamide gel electrophoresis.

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Figure 1. Comparison of DNA detection in 1% agarose gel in TBE buffer using Gelite™ Safe, EtBr, and SYBR® Safe. Two-fold serial dilutions of 1 kb DNA ladder were loaded in amounts of 100 ng, 50 ng, and 25 ng from left to right. Gels were stained for 60 minutes with Gelite™ Safe, EtBr, and SYBR® Safe according to the manufacturer's recommended concentrations and imaged using the ChemiDoc™ Imaging System (Bio-Rad®). Gels were illuminated using a 300 nm transilluminator fitted with a GelGreen filter.

Figure 2. Comparison of DNA detection in 1% agarose gel in TBE buffer using Gelite™ Safe, SYBR® Safe, and EtBr. Two-fold serial dilutions of 1 kb DNA ladder were loaded in amounts of 86 ng, 43 ng, 21.5 ng, 10.7 ng, 5.3 ng, 2.6 ng, 1.3 ng, and 0.5 ng from left to right. Gels were imaged using a 300 nm transilluminator in ChemiDoc™ Imaging System (Bio-Rad®).

Gelite™ Safe DNA Gel Stain. Gelite™ Safe exhibits a considerable fluorescence enhancement by several orders of magnitude greater than that of EtBr. Gelite™ Safe was optimized to be compatible with various instruments, including UV and blue-light transiluminators, gel documentation systems, and laser scanners. It is the first single formulation that can be used in either the green or red channel at your preference. Unlike the membrane-permeant SYBR® Green, which is highly toxic to cells and the environment, the membrane-impermeant properties of Gelite™ Safe make it a much safer and noncytotoxic alternative.

Transilluminator

Excitation: 254 nm or 300 nm

Emission: Long path green filter (ex. SYBR or GelStar)

 

Product

Ex (nm)¹

Filter²

Unit Size

Cat No.

Gelite™ Green Nucleic Acid Gel Staining Kit

254 nm or 300 nm

Long path green filter

1 Kit

17589

Gelite™ Orange Nucleic Acid Gel Staining Kit

254 nm or 300 nm

Long path green filter

1 Kit

17594

Gelite™ Safe DNA Gel Stain *10,000X DMSO Solution*

254 nm, 300 nm or 520 nm

Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters

100 µL

17704

Gelite™ Safe DNA Gel Stain *10,000X DMSO Solution*

254 nm, 300 nm or 520 nm

Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters

500 µL

17705

Gelite™ Safe DNA Gel Stain *10,000X DMSO Solution*

254 nm, 300 nm or 520 nm

Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters

1 mL

17706

Gelite™ Safe DNA Gel Stain *10,000X DMSO Solution*

254 nm, 300 nm or 520 nm

Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters

10 mL

17707

Gelite™ Safe DNA Gel Stain *10,000X Water Solution*

254 nm, 300 nm or 520 nm

Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters

100 µL

17700

Gelite™ Safe DNA Gel Stain *10,000X Water Solution*

254 nm, 300 nm or 520 nm

Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters

500 µL

17701

Gelite™ Safe DNA Gel Stain *10,000X Water Solution*

254 nm, 300 nm or 520 nm

Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters

1 mL

17702

Gelite™ Safe DNA Gel Stain *10,000X Water Solution*

254 nm, 300 nm or 520 nm

Ethidium Bromide, Gel Star, Gel Green, Gel Red and SYBR filters

10 mL

17703

Helixyte™ Gold Nucleic Acid Gel Stain *10,000X DMSO Solution*

254 mn

Long path green filter

1 mL

17595

Helixyte™ Green Nucleic Acid Gel Stain *10,000X DMSO Solution*

254 mn

Long path green filter

1 mL

17590

Helixyte™ Green Nucleic Acid Gel Stain *10,000X DMSO Solution*

254 mn

Long path green filter

100 µL

1760

Helixyte™ Green Fluorimetric DNA Quantitation Kits for use with fluorescence microplate readers

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figure-for-helixyte-green-fluorimetric-dsdna-quantitation-kit-optimized-for-microplate-readers_ekdvl

Figure 1. Comparison of dsDNA dose response using the Helixyte Green™ (blue ) with InvitrogenTM Quant-iT™ PicoGreen® dsDNA Reagent (red ). dsNDA standards were incubated in cuvettes and measured using varian cary eclipse fluorescence spectrophotometer. 

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Helixyte™ Green is an excellent nucleic acid sensor that exhibits large fluorescence enhancement upon binding to dsDNA. It has the same spectral properties to those of PicoGreen®, thus a great replacement to PicoGreen® (PicoGreen® is the trademark of Invitrogen).

Fluorescence microplate reader

Excitation: 490 nm

Emission: 525 nm

Cutoff: 515 nm

Recommended plate: Solid black

Product

Ex/Em (nm)

Target

Detection Range

Unit Size

Cat No.

Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *High Sensitivity*

490/525

dsDNA

0.2-100 ng

200 tests

17651

Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *Optimized for Broad Dynamic Range*

490/530

dsDNA

2-1000 ng

200 tests

17645

Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *Optimized for Broad Dynamic Range*

490/530

dsDNA

2-1000 ng

1000 tests

17646

Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *Optimized for Microplate Readers*

490/525

dsDNA

0.2-100 ng

200 tests

17650

Helixyte™ Green Fluorimetric ssDNA Quantitation Kit *Optimized for Microplate Readers*

490/525

ssDNA

1-200 ng

200 tests

17623

Helixyte™ Green Fluorimetric Total Nucleic Acid Quantitation Kit *Optimized for Microplate Readers*

490/525

dsDNA, ssDNA, RNA, oligos

as little as 0.3 ng

200 tests

17630

1280px-Berthold_logo.svg

Tristar Multimode Plate Readers 
Flexibility and sensitivity whenever you need it

Introducing the NEW Tristar family of modular multimode plate readers from Berthold, Germany. Configured ready for fluorescence and UV/vis nucleic quantification. With life time application support and flexible configuration, for when you need a new assay setup.

Tristar31080

Introducing the NEW Tristar 3

Simplicity and sensitivity for all levels of experience

Learn more ›

Tristar51080

Introducing the NEW Tristar 5

Flexibility and sensitivity whenever you need it

Learn more ›

Portelite™ Fluorimetric Nucleic Acid Quantitation Kits for use with the CytoCite™ and Qubit® fluorometers

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Figure 1. DNA standard curve generated using Portelite™ Fluorimetric DNA Quantitation Kit with Broad Dynamic Range. Fluorescence intensity was quantified using green fluorescence channel, regression model was calculated using linear fit.

DNA Quantitation is a very important task in DNA sample preparations for various analyses. Portelite™ Fluorimetric DNA Quantitation Kit provides a rapid method to quantify dsDNA with Helixyte™ Green BR using a hand-held fluorometer. It is optimized for Cytocite™ and Qubit™ fluorometers. Portelite™ Fluorimetric DNA Quantitation assay is linear over three orders of magnitude. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is optimized for measuring dsDNA concentrations from 10 pg/µL to 10 ng/µL. Helixyte™ Green BR exhibits large fluorescence enhancement upon binding to dsDNA, and it is a few magnitudes more sensitive than UV absorbance readings.

Qubit Fluorometer
Excitation: 480 nm
Emission: 520 nm
Instrument specification(s): 0.2 mL, thin-wall PCR tube

CytoCite Fluorometer
Excitation: 480 nm
Emission: 520 nm
Instrument specification(s): 0.2 mL, thin-wall PCR tube

Product

Platform

Ex/Em (nm)

Target

Detection Range

Unit Size

Cat No.

Portelite™ Fluorimetric DNA Quantitation Kit with Broad Dynamic Range

CytoCite™ BG100 or Qubit® Fluorometer

480/530

dsDNA

2-1000 ng

100 tests

17665

Portelite™ Fluorimetric High Sensitivity DNA Quantitation Kit

CytoCite™ BG100 or Qubit® Fluorometer

480/520

dsDNA

0.2-100 ng

100 tests

17660

Portelite™ Fluorimetric High Sensitivity DNA Quantitation Kit

CytoCite™ BG100 or Qubit® Fluorometer

480/520

dsDNA

0.2-100 ng

500 tests

17661

Portelite™ Fluorimetric RNA Quantitation Kit

CytoCite™ BG100 or Qubit® Fluorometer

480/530

RNA

5-100 ng

100 tests

17658

Portelite™ Fluorimetric RNA Quantitation Kit

CytoCite™ BG100 or Qubit® Fluorometer

480/530

RNA

5-100 ng

500 tests

17659

Portelite™ Fluorimetric ssDNA Quantitation Kit

CytoCite™ BG100 or Qubit® Fluorometer

480/530

ssDNA

1-200 ng

200 tests

17625

Portelite™ Fluorimetric Total Nucleic Acid Quantitation Kit

CytoCite™ BG100 or Qubit® Fluorometer

480/530

dsDNA, ssDNA, RNA, oligos

as little as 0.3 ng

200 tests

17631

Portelite™ runs on all Qubit tube fluorimeters

Please contact our friendly team to assist with product selection, pricing and delivery information. Freephone 0800 724 633 or email info@scienze.nz 

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CytoCite™ Fluorometer
Fast, Specific & Accurate Quantitation

Fast, Specific & Accurate Quantitation in a small benchtop form fluorimeter, sample volume's down to 150uL...

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Freephone 0800 724 633

info@scienze.nz

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