Fast, Simple and Accurate

Protein Quantification

Fast, Simple and Accurate

Protein Quantification

Protein Quantification Instruments ›

Protein Quantification Kits ›

Protein Quantification Kits

Click the links below to learn more

Catalogue No.: 11100 Amplite™; Fluorimetric Fluorescamine Protein Quantitation Kit *Blue Fluorescence* 200 Tests

Catalogue No.: 11105 Amplite™; Fluorimetric Fluorescamine Protein Quantitation Kit *Orange Fluorescence* 500 Tests

Catalogue No.: 11109 Portelite™; Fluorimetric Protein Quantitation Kit *Optimized for CytoCite™; and Qubit™; Fluorometers* 100 Tests

Catalogue No.: 11111 Portelite™; Fluorimetric Protein Quantitation Kit *Optimized for CytoCite™; and Qubit™; Fluorometers* 100 Tests

Catalogue No.: 11115 Amplite™ Colorimetric BCA Protein Quantitation Assay Kit 1000 Tests

Catalogue No.: 11116 Amplite™ Colorimetric BCA Protein Quantitation Assay Kit 5000 Tests

Catalogue No.: 11118 Amplite™; Colorimetric Bradford Protein Quantitation Assay Kit 1000 Tests

Catalogue No.: 11119 Amplite™; Colorimetric Bradford Protein Quantitation Assay Kit 5000 Tests

Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.

Biuret, Lowry, BCA and Bradford assays are routinely used for estimating protein concentration. However, these colorimetric assays are less sensitive, and require large sample volume to ensure accuracy.

Our Portelite™ Fluorimetric Protein Quantitation Kit is significantly more sensitive than existing colorimetric protein measurements, e.g., Bradford and Bicinchoninic acid (BCA) assays.

Prolite™ Orange used in the kit is non-fluorescent in aqueous solution, but reacts rapidly with proteins and generates bright fluorescence.

The Portelite™ Fluorimetric Protein Quantitation Kit provides a simple method for quantifying protein concentration in solutions. The assay has dynamic range from 12.5 ug/mL to 5 mg/mL of BSA.

The kit is optimized for Cytocite™ and Qubit™ fluorometers.

It can be used for (1) studying protein/protein interactions; (2) measuring column fractions after affinity chromatography; (3) estimating recovery of membrane proteins from cell extract; and (4) high-throughput screening of fusion proteins.

Protein Quantification Instruments

Scienze offers three types of instruments used for the spectrophotometric determination of proteins.

There is the CytoCite single channel Fluorometer, the NEW Berthold Colibri+ microvolume spectrophotometer and the NEW Tristar family of microplate readers to suit your sample volume & sample number requirements.