Tristar5500

Tristar 5

Multimode Microplate Reader

Flexibility and sensitivity whenever you need it

Flexibility and sensitivity whenever you need it

The Tristar 5 is a modular high-performance microplate reader equipped with independent, user-selectable filters and monochromators on both, the excitation and emission side for any measurement. This guarantees both, flexibility and sensitivity whenever you need it. The system supports advanced detection modes such as TRF and TR-FRET, and FP as well as specific assays like BRET/BRET2, NanoBRET™ , Lantha-Screen® or AlphaScreen®.

Detection Technologies

The Tristar 5 Multimode Microplate Reader provides you with application flexibility for today, tomorrow, and beyond to master your changing plate reading applications. The system supports the following wide range of detection modes:

  • Absorbance (UV/VIS)
  • Fluorescence Intensity (including FRET)
  • Luminescence (including BRET and BRET2)
  • Time Resolved Fluorescence (TRF)
  • Time Resolved FRET (TR-FRET / HTRF®)
  • Fluorescence Polarization
  • AlphaScreen®

Detection Technologies

The Tristar 5 Multimode Microplate Reader provides you with application flexibility for today, tomorrow, and beyond to master your changing plate reading applications. The system supports the following wide range of detection modes:

  • Absorbance (UV/VIS)
  • Fluorescence Intensity (including FRET)
  • Luminescence (including BRET and BRET2)
  • Time Resolved Fluorescence (TRF)
  • Time Resolved FRET (TR-FRET / HTRF®)
  • Fluorescence Polarization
  • AlphaScreen®
Detection-technologies-new
FlexTec-opticsTristar5

FlexTec Optics – when flexibility AND sensitivity count

The Tristar 5 system is equiped with FlexTec Optics, offering you the best of two worlds – benefit from the flexibility to easily select a discrete wavelength and perform spectral scans for both, excitation and emission using its built-in monochromator technology. Or optimize your assays´ sensitivity by utilizing the system filter sliders for excitation and emission. Or mix both technologies if required: the Tristar 5 delivers both, flexibility and sensitivity in a single system.

The Tristar 5 employs up to two double monochromators providing blocking properties needed in sensitive fluorescence assays. Both monochromators are equipped with software driven continuous bandwidth variation to optimise the instrument for the specific demands of different assay conditions.

Berthold´s flexible monochromator technology offers you variable bandwidths from 4-12 nm in excitation and 8 – 22 nm for emission, selectable in 1 nm increments.

FlexTec Optics – when flexibility AND sensitivity count

The Tristar 5 system is equiped with FlexTec Optics, offering you the best of two worlds – benefit from the flexibility to easily select a discrete wavelength and perform spectral scans for both, excitation and emission using its built-in monochromator technology. Or optimize your assays´ sensitivity by utilizing the system filter sliders for excitation and emission. Or mix both technologies if required: the Tristar 5 delivers both, flexibility and sensitivity in a single system.

The Tristar 5 employs up to two double monochromators providing blocking properties needed in sensitive fluorescence assays. Both monochromators are equipped with software driven continuous bandwidth variation to optimise the instrument for the specific demands of different assay conditions.

Berthold´s flexible monochromator technology offers you variable bandwidths from 4-12 nm in excitation and 8 – 22 nm for emission, selectable in 1 nm increments.

Broad wavelength range selection from UV through the visible range

From UV absorbance for DNA quantification or UV excitation for tryptophan fluorescence, to measurements in the far red or NIR (in selected configurations), the Tristar 5 is ready for any assay .

Broad wavelength range selection from UV through the visible range

From UV absorbance for DNA quantification or UV excitation for tryptophan fluorescence, to measurements in the far red or NIR (in selected configurations), the Tristar 5 is ready for any assay .

Advanced bottom reading technology - enhanced cell-based assay performance

The Tristar 5 enables you to read both, from below as well as from the top with high-performance. The advanced bottom-reading technology of the system can be easily selected via the software to help you analyse e.g. adherent cells and obtain best sensitivity and a superior signal-to-background ratio for these applications.

Advanced bottom reading technology - enhanced cell-based assay performance

The Tristar 5 enables you to read both, from below as well as from the top with high-performance. The advanced bottom-reading technology of the system can be easily selected via the software to help you analyse e.g. adherent cells and obtain best sensitivity and a superior signal-to-background ratio for these applications.

ONE-4-ALL Optics - uncompromised performance

Our patented ONE-4-ALL optics have been optimised to combine the stability and user-friendliness of a multimodal optical system with the sensitivity and versatility of dedicated optical devices.

ONE-4-ALL optics work for both filter- and monochromator-based applications, so there´s never a compromise in performance in any mode.

ONE-4-ALL Optics - uncompromised performance

Our patented ONE-4-ALL optics have been optimised to combine the stability and user-friendliness of a multimodal optical system with the sensitivity and versatility of dedicated optical devices.

ONE-4-ALL optics work for both filter- and monochromator-based applications, so there´s never a compromise in performance in any mode.

One-4-ALL-960
Tristar3-filters

High-performance filter system - when sensitivity counts

The quick-change filter technology of the Tristar series provides you with the flexibility required to meet your application needs: up to 40 different excitation and emission filters can be easily mounted on exchangeable filter slides.

The filters are characterized by high transmissions properties which can be up to 25-fold that of monochromators. Technologies like Time-Resolved Fluorescence (TRF) can be measured more efficiently with filters.

Furthermore, filters with a large bandwidth are available to analyze fluorophores with wide spectra and all luminescence-based assay requiring to use filters, e.g. BRET, BRET2 and NanoBRET™ assays.

High-performance filter system - when sensitivity counts

The quick-change filter technology of the Tristar series provides you with the flexibility required to meet your application needs: up to 40 different excitation and emission filters can be easily mounted on exchangeable filter slides.

The filters are characterized by high transmissions properties which can be up to 25-fold that of monochromators. Technologies like Time-Resolved Fluorescence (TRF) can be measured more efficiently with filters.

Furthermore, filters with a large bandwidth are available to analyze fluorophores with wide spectra and all luminescence-based assay requiring to use filters, e.g. BRET, BRET2 and NanoBRET™ assays.

Ergonomic design to support your work

The ergonomic design of the system provides full front access for all key operations (e.g. plate loading, filter change and connecting reagents). Reagent vials can be stored in an integrated front compartment, providing easy access and visibility. It contains a removable trough that can be filled with water or ice to keep all reagents cooled. Different reagent tube holders are available to ensure secure handling of valuable reagents. The system has a flat surface on its top, providing enough space to put down a laptop.

Ergonomic design to support your work

The ergonomic design of the system provides full front access for all key operations (e.g. plate loading, filter change and connecting reagents). Reagent vials can be stored in an integrated front compartment, providing easy access and visibility. It contains a removable trough that can be filled with water or ice to keep all reagents cooled. Different reagent tube holders are available to ensure secure handling of valuable reagents. The system has a flat surface on its top, providing enough space to put down a laptop.

Tristar3-reagents
Crosstalk-blue

Powerful crosstalk control – reliable results

Do you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples?  Then you´re potentially affected by a phenomenon called crosstalk, that can produce wrong results and, hence, wrong conclusions. The crosstalk reduction design of the Tristar 5 does almost eliminate this problem: with a crosstalk of 0.000001, you never have to worry about the position of your high-intensity samples in your plate.

Powerful crosstalk control – reliable results

Do you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples?  Then you´re potentially affected by a phenomenon called crosstalk, that can produce wrong results and, hence, wrong conclusions. The crosstalk reduction design of the Tristar 3 does almost eliminate this problem: with a crosstalk of 0.000001, you never have to worry about the position of your high-intensity samples in your plate.

JET injectors: highest accuracy, speed and cell-friendliness

The Tristar 3 can be equipped with up to 3 JET injectors with variable volumes in pre-measurement or measurement position. The ultra-fast injection speed of JET injectors eliminates any delay between injection and measurement and enables measurement of fast reactions, e.g. flash luminescence.

Berthold Technologies' JET injectors use Teflon bellows for accurate and fast injections and guarantee most efficient mixing as well as extreme longevity.

  • Highest accuracy and reproducibility - 98 % accuracy over the entire volume range
  • Low reagent consumption - Low dead volume. Recovers up to 60 % of the reagent in the injector line. A single priming sequence is sufficient to achieve a 99 % homogeneous mix at the tip.
  • Low maintenance costs – friction-free operation enables more than 3 million injections without mechanical failure.
  • Optimal performance in flash-kinetics - ultra-fast injection enables the measurement of the first 150 ms of a kinetic assay (e.g. Fura-2 or Acridiniumester-assys).
  • Worry-free injection of cell suspensions - negligible shear forces ideal when working with living cells, e.g. in Aequorin-based calcium assays

JET injectors: highest accuracy, speed and cell-friendliness

The Tristar 3 can be equipped with up to 3 JET injectors with variable volumes in pre-measurement or measurement position. The ultra-fast injection speed of JET injectors eliminates any delay between injection and measurement and enables measurement of fast reactions, e.g. flash luminescence.

Berthold Technologies' JET injectors use Teflon bellows for accurate and fast injections and guarantee most efficient mixing as well as extreme longevity.

  • Highest accuracy and reproducibility - 98 % accuracy over the entire volume range
  • Low reagent consumption - Low dead volume. Recovers up to 60 % of the reagent in the injector line. A single priming sequence is sufficient to achieve a 99 % homogeneous mix at the tip.
  • Low maintenance costs – friction-free operation enables more than 3 million injections without mechanical failure.
  • Optimal performance in flash-kinetics - ultra-fast injection enables the measurement of the first 150 ms of a kinetic assay (e.g. Fura-2 or Acridiniumester-assys).
  • Worry-free injection of cell suspensions - negligible shear forces ideal when working with living cells, e.g. in Aequorin-based calcium assays
jet-injector-linearity-new
ICE-MW-test

Convenient Software options

Choose between different software options according to your application needs:

ICE Software

ICE software is an intuitive, wizard-guided software that walks you through the entire process of setting up your system, collecting your data and reporting the results. The software package is as flexible as your research thanks to the many setting options and freely configurable combinations of operating sequences.

MikroWin Software

The MikroWin software is a complete software solution providing instrument control, data collection, analysis and reporting. The software is available in different versions, reflecting different user needs. The complete Advance version of the software provides 21 CFR Part 11 compliance and additional QC features like audit trail or configurable user hierarchies.

Convenient Software options

Choose between different software options according to your application needs:

ICE Software

ICE software is an intuitive, wizard-guided software that walks you through the entire process of setting up your system, collecting your data and reporting the results. The software package is as flexible as your research thanks to the many setting options and freely configurable combinations of operating sequences.

MikroWin Software

The MikroWin software is a complete software solution providing instrument control, data collection, analysis and reporting. The software is available in different versions, reflecting different user needs. The complete Advance version of the software provides 21 CFR Part 11 compliance and additional QC features like audit trail or configurable user hierarchies.

Validation tools, Service & Support

For many laboratories, validation, qualification and ensuring compliance with a number of GMP and GLP requirements is essential. We provide a number of tools and services to help you ensure that your system is running at peak performance.

Validation Tools

We provide a variety of tools to help you check and confirm the performance of your products over time.

Service

Berthold Expert Services provide a team of dedicated and factory-trained engineers and experts to optimize your productivity. We and our local partners are always at your disposal.

Support

Our team of technical support scientists is your partner to overcome the unique challenges your application brings. Contact our team to discuss your assay or experiment design, data analysis questions, troubleshooting and more.

Validation tools, Service & Support

For many laboratories, validation, qualification and ensuring compliance with a number of GMP and GLP requirements is essential. We provide a number of tools and services to help you ensure that your system is running at peak performance.

Validation Tools

We provide a variety of tools to help you check and confirm the performance of your products over time.

Service

Berthold Expert Services provide a team of dedicated and factory-trained engineers and experts to optimize your productivity. We and our local partners are always at your disposal.

Support

Our team of technical support scientists is your partner to overcome the unique challenges your application brings. Contact our team to discuss your assay or experiment design, data analysis questions, troubleshooting and more.

ScienzeTristarValidation
Tristar 3 and Tristar 5 Brochure

Brochure of the Tristar 3 and Tristar 5 Multimode Microplate Readers.

Download Brochure ›

Tristar Family Application Notes

The following Application Notes were created with earlier instrument versions of the Tristar system family, but can be transferred 1:1 to the new Tristar systems.

Transcreener® Fluorescence Intensity Assay with the TriStar² S The compatibility of the Transcreener® Fluorescence Intensity Assay Kit for ADP is tested with the TriStar² S LB 942 Multimode Microplate Reader.

Download Application Note ›

Transcreener® GDP TR-FRET Red Assay with TriStar² S Validation of the TriStar² S Multimode Microplate Reader with the Transcreener® GDP TR-FRET Red assay.

Download Application Note ›

NanoBRET™ with the TriStar² S Promega NanoBRET™ protein:protein interaction system with the TriStar² S Multimode Microplate Reader

Download Application Note ›

Screening of GPCR antagonists using Ca2+ measurements and the TriStar² S3 commercially available compounds are screened for P2Y2 receptor inhibition using the calcium mobilisation assay with two different fluorescent dyes measured on a TriStar² LB 942 multimode microplate reader.

Download Application Note ›

Monitoring circadian gene expression in micro-algae using the TriStar²We report how the Berthold TriStar² multimode microplate reader equipped with luminescence module, in combination with an external LED illumination adaptor, can be used to image circadian rhythms in the micro algae Ostreococcus tauri.

Download Application Note ›

Tristar5390

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