kikkomann500

Microbiological Testing

Microbiological Testing

A prepared film medium that does not require medium preparation.

img_ep_ac
img_ep_cc
img_ep_ec
img_ep_sa

It does not require medium preparation or autoclave sterilization and can be used immediately after taking it out of the bag. Work time can be shortened because there is no need for operations such as placing or layering or special jigs. Since it can be dropped while stacking, not only the work time but also the work space can be reduced. Easy operation that even beginners can use with high reliability. 

img_ep_ac2

Easy Plate AC

General viable bacteria

 Count the number of red colonies

img_ep_cc2

Easy Plate CC

Coliform bacteria

 Count the number of blue colonies

img_ep_ec2

Easy Plate EC

E. coli 

Count the number of dark blue to bluish purple colonies [Coliform bacteria other than E. coli] Count the number of pink to purplish red colonies

img_ep_sa2

Easy Plate SA

Staphylococcus aureus

 Count the number of blue colonies

Easy to Use

Easy to Use

1. Sample Preparation

Add appropriate sterile dilutions to the sample and homogenize with a homogenizer.

Dilute the sample solution so that the number of bacteria is within an appropriate range ( 250cfu/ml or less )

2. Inoculation

tear-off-the-foil-pouch

Aseptically remove each sheet form the aluminium bag.

placed-on-a-flat-position

Place sheet on a flat surface and lift the cover.

open-the-tectorial-membrane

Place a 1ml sample suspension onto the centre of the medium.

overlay-tectorial-membrane

Leave the sheet on a flat, horizontal surface for 3 minutes or more.

Increase efficiency by stacking 

Efficiency1
Efficiency2
Efficiency3

New sheets can be stacked on top of sheets in which solidification is still occurring ( up to 10 sheets ). This saves space and time during sample placement.

3. Incubation

put-into-the-incubator

Incubate the sheets, in lots of up to 25 sheets.

4. Interpretation

Interpretation1

The use of chromogens assist in the visualization of bacterial colonies and counting.

For a Large Number of Colonies

Count colonies within 1-3 squares printed on the cover and calculate an average. 

Colony Isolation

Lift the cover and pick a single colony from the gel.

multiply-the-bacteria-number

5. Disposal

All used sheets should be considered to be contaminated and should be disposed of it in accordance with the disposal standards of local governments and facilities after  appropriate sterilization.

AOAC Research Institute Search Performance Tested Methods

As of April 1, 2021, manufacturing and sales of the film media ‘’Medi・Ca’’ is transferred to Kikkoman Biochemifa Company.

Type: Microbiological
Test Kit Name: Medi Ca AC
Manufacturer: Dai Nippon Printing Co., Ltd.
License: 041302
Extra Certification: n/a
Catalog Number: AC-01
Analytes: Aerobic bacteria
Matrixes: Matrix Claim

 

Type: Microbiological
Test Kit Name: Medi Ca CC
Manufacturer: Dai Nippon Printing Co., Ltd.
License: 021401
Extra Certification: n/a
Catalog Number: CC-01
Analytes: Coliform bacteria
Matrixes: Matrix Claim

 

Type: Microbiological
Test Kit Name: Medi Ca EC
Manufacturer: Dai Nippon Printing Co., Ltd.
License: 031601
Extra Certification: n/a
Catalog Number: EC-01
Analytes: E. coli and coliform bacteria
Matrixes: beef, pork, frozen pork, lamb, salmon, frankfurter sausage, ham

Type: Microbiological
Test Kit Name: Medi Ca SA
Manufacturer: Dai Nippon Printing Co., Ltd.
License: 111703
Extra Certification: n/a
Catalog Number: n/a
Analytes: Staphylococcus aureus
Matrixes: raw beef, raw ground beef, raw lamb, cooked ham, raw salmon, frozen prawn,
fresh chilled pasta, pasteurized milk, natural cheese, cream puff and potato salad

ColonyCounter800

Looking for a Colony Counter?

Learn more ›

Need more information / Questions?

Need more information / Questions?

Please contact us by phone or email:

Freephone 0800 724 633

info@scienze.nz

Or use the contact form below:

Please enter your name.
Please enter a message.

For Greater Productivity & Insights

For Greater Productivity & Insights

Copyright © 2021, Scienze. All rights reserved.