Ultrasensitive, Reliable and Accurate Luminescence Measurement

Centro LB 963 Microplate Luminometer

The Centro is a high-performance, easy-to-use and versatile 96-well microplate luminometer for both, glow and flash luminescence assays. The optimized optical system of the instrument provides lowest background and negligible crosstalk (10^-6). It can be equipped with up to 3 injectors.

The Centro XS version of the system has been built on the basis of the successful Centro, and shares with it its reliability and robustness, but has been optimized to increase its sensitivity even further: the Centro XS is the most sensitive microplate luminometer available and reliably measures less than 1.8 amol ATP per well. This makes the Centro XS the ideal partner for applications such as determination of cell viability, e.g. in tumour chemosensitivity assay, cell proliferation, antibiotic susceptibility testing or pathogen detection.

The Centro supports all basic luminescence technologies including:

Flash-type Luminescence
Glow-type Luminescence
Cellular Luminescence
Bio-Luminescence
Chemi-Luminescence

Sensitive and reliable

Thanks to a stringent selection of photomultipliers working in photon counting mode and more than 40 years of experience building luminometers, the Centro provides highly sensitive luminescence detection: less than 5 amol ATP/well (25 fM) can be detected reliably. In the Centro XS, optics have been optimized to increase its sensitivity even further: below 1.8 amol ATP/well (9 fM).

Advanced Crosstalk reduction

With the Centro, intense samples don’t interfere with weak ones! Berthold Technologies has patented designs to eliminate signals from not-measured wells.

Berthold crosstalk specifications are 10^-6.

This equates to 1,000,000 counts in a particular well and only 1 count in all of the adjacent wells.

Integrated waste pump

Thanks to its built-in waste pump, you don’t need to worry about loading microplates, waste containers or removal of injector tips for washing and filling the reagent lines; the built-in waste pump takes care of the safe removal of excess liquids.

JET injectors: highest accuracy, speed and cell-friendliness

JET injectors: highest accuracy, speed and cell-friendliness

The Centro can be equipped with up to 3 JET injectors with variable volumes in pre-measurement or measurement position*. The ultra-fast injection speed of JET injectors eliminates any delay between injection and measurement and enables measurement of fast reactions, e.g. flash luminescence.

Berthold Technologies' JET injectors use Teflon bellows for accurate and fast injections and guarantee most efficient mixing as well as extreme longevity.

Accurate pipetting of small volumina: JET injectors ensure the injection of large and small volumes with high accuracy and offer a precision of more than 98% over the entire volume range
Low maintenance costs: frictionless operation for extended lifetime
Cell-friendly performance: cell-friendly materials and negligible shear forces enable worry-free injection of cell suspensions, e.g. in Aequorin-based calcium assays

* All injectors in pre-measurement position in the Centro XS.

Up to 3 injectors
10 to 100µl
1µL increments
Can be unloaded to recover reagent
Customizeable priming
Tips in measurement position for 96 and 384 well plates

Convenient Software options

Choose between different software options according to your application needs:

ICE Software

ICE software is an intuitive, wizard-guided software that walks you through the entire process of setting up your system, collecting your data and reporting the results. The software package is as flexible as your research thanks to the many setting options and freely configurable combinations of operating sequences.

MikroWin Software

The MikroWin software is a complete software solution providing instrument control, data collection, analysis and reporting. The software is available in different versions, reflecting different user needs. The complete Advance version of the software provides 21 CFR Part 11 compliance and additional QC features like audit trail or configurable user hierarchies.

Technical Details

Measurement

Injectors

Microplates

Environmental Control

Performance

Operation

Additional Specifications

Regulations: CE, NRTL

Power supply: 100 – 240 VAC ±10%, 50/60 Hz

Operating Voltage: 24 VDC / 6.67 A

Temperature tolerance: Storage: 0-40° C
Operation: 15-35° C

Humidity tolerance: 10-80% non-condensing
Maximum relative humidity of 80 % for temperatures up to 31 °C; decreasing linearly to 50 %, relative humidity up to 40 °C

Dimensions (W x D x H): 346.8 x 287.5 x 443 mm

Weight: 21.8kg

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Berthold's comprehensive line of reliable Microplate Readers deliver the versatility, sensitivity, precision, dynamic range and throughput you need to solve your complex analytical challenges. Every lab is unique. That's why you deserve a Microplate Reader that fits your needs. Maybe your research requires maximum application flexibility, or high throughput. Perhaps you are looking for simplicity, delivering reliable results. Whatever you need, there is a Berthold Technologies Microplate Reader to fit your research needs.