LysoBrite Dyes Lysosomal Staining Probes
LysoBrite Dyes Lysosomal Staining Probes

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LysoBrite Image Example

Images above of HeLa cells stained with AAT’s LysoBrite ™ Green (left), and Invitrogen’s LysoTracker® Green DND-26 (right) in a Costar black wall/clear bottom 96-well plate. Samples were continuously illuminated for 120 seconds, and the signals were compared before and after the exposure by using a Keyence fluorescence microscope.

LysoBrite™ Dye Range

Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. Find the following range of LysoBrite™Dyes:

LysoBrite™ Dyes Safety Data Sheet

For more information please email info@scienze.nz  and one of our technical specialists will contact you directly to discuss your needs.

LysoBrite™ NIR Overview

Catalog Number:
22641
Unit Size:
500 Tests

 

Molecular weight Excitation (nm) Emission (nm)
730.89 634 649

 

Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains this pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels. LysoBrite™ NIR selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. LysoBrite™ dyes significantly outperform the equivalent LysoTracker ™dyes (from Invitrogen). LysoBrite™ dyes can stay in live cells for more than a week with very minimal cell toxicity while the LysoTracker dyes can only be used for a few hours.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of LysoBrite™ NIR to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg 0.5 mg 1 mg 5 mg 10 mg
1 mM 136.819 µL 684.097 µL 1.368 mL 6.841 mL 13.682 mL
5 mM 27.364 µL 136.819 µL 273.639 µL 1.368 mL 2.736 mL
10 mM 13.682 µL 68.41 µL 136.819 µL 684.097 µL 1.368 mL

 

Figure 1. Image of HeLa cells stained with Cell Navigator™ Lysosome Staining Kit in a Costar black wall/clear bottom 96-well plate.

LysoBrite™ Green Overview

Catalog Number:
22643
Unit Size:
500 Tests

 

Molecular weight Excitation (nm) Emission (nm)
453.34 500 509

 

Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains this pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels. LysoBrite™ Green selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of LysoBrite™ Green to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg 0.5 mg 1 mg 5 mg 10 mg
1 mM 220.585 µL 1.103 mL 2.206 mL 11.029 mL 22.058 mL
5 mM 44.117 µL 220.585 µL 441.17 µL 2.206 mL 4.412 mL
10 mM 22.058 µL 110.292 µL 220.585 µL 1.103 mL 2.206 mL

Figure 1. Images of HeLa cells stained with A: AAT’s LysoBrite ™ Green, B: Invitrogen’s LysoTracker® Green DND-26 in a Costar black wall/clear bottom 96-well plate. Samples were continuously illuminated for 120 seconds, and the signals were compared before and after the exposure by using a Keyence fluorescence microscope.

LysoBrite™ Red Overview

Catalog Number:
22645
Unit Size:
500 Tests

 

Molecular weight Excitation (nm) Emission (nm)
698.94 575 595
Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains this pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels. LysoBrite™ Red selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. LysoBrite™ dyes significantly outperform the equivalent LysoTracker ™dyes (from Invitrogen). LysoBrite™ dyes can stay in live cells for more than a week with very minimal cell toxicity while the LysoTracker dyes can only be used for a few hours. LysoBrite™ dyes can survive a few generations of cell division. In addition, LysoBrite™ dyes are much more photostable than the LysoTracker dyes.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of LysoBrite™ Red to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg 0.5 mg 1 mg 5 mg 10 mg
1 mM 143.074 µL 715.369 µL 1.431 mL 7.154 mL 14.307 mL
5 mM 28.615 µL 143.074 µL 286.148 µL 1.431 mL 2.861 mL
10 mM 14.307 µL 71.537 µL 143.074 µL 715.369 µL 1.431 mL

Figure 1. Images of HeLa cells stained with A: LysoBrite ™ Red, B: LysoTracker® Red DND-99 (from Invitrogen) in a Costar black wall/clear bottom 96-well plate. Samples were continuously illuminated for 120 seconds, and the signals were compared before and after the exposure by using an Olympus fluorescence microscope.

 

Figure 2. Lysosome localization and motility is altered for starvation-induced Hela cells. A: Healthy untreated  Hela cells. Lysosomes (Red) were dispersed widely throughout the cytosol in cells.  B: Starved Hela Cells. The cells were starved for 24 hours (no serum), and lysosomes were aggregated in the perinuclear region. Nuclei were stained with Hoechst 33342.

 

Figure 3. Image of Hela cells stained with Cell Navigator™ Lysosome Staining Kit in a Costar black wall-clear bottom 96-well plate.

LysoBrite™ Blue Overview

Catalog Number:
22642
Unit Size:
500 Tests

 

Molecular weight Excitation (nm) Emission (nm)
331.42 432 479

 

Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains this pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels. LysoBrite™ Blue selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of LysoBrite™ Blue to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg 0.5 mg 1 mg 5 mg 10 mg
1 mM 301.732 µL 1.509 mL 3.017 mL 15.087 mL 30.173 mL
5 mM 60.346 µL 301.732 µL 603.464 µL 3.017 mL 6.035 mL
10 mM 30.173 µL 150.866 µL 301.732 µL 1.509 mL 3.017 mL

Figure 1. Image of HeLa cells stained with Cell Navigator™ Lysosomal Staining Kit in a Costar black wall/clear bottom 96-well plate.

 

LysoBrite™ Orange Overview

Catalog Number:
22644
Unit Size:
500 Tests

 

Molecular weight Excitation (nm) Emission (nm)
670.89 542 564

 

Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains this pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels. LysoBrite™ Orange selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. LysoBrite™ dyes significantly outperform the equivalent LysoTracker ™dyes (from Invitrogen). LysoBrite™ dyes can stay in live cells for more than a week with very minimal cell toxicity while the LysoTracker dyes can only be used for a few hours. LysoBrite™ dyes are much more photostable than the LysoTracker dyes.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of LysoBrite™ Orange to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg 0.5 mg 1 mg 5 mg 10 mg
1 mM 149.056 µL 745.279 µL 1.491 mL 7.453 mL 14.906 mL
5 mM 29.811 µL 149.056 µL 298.111 µL 1.491 mL 2.981 mL
10 mM 14.906 µL 74.528 µL 149.056 µL 745.279 µL 1.491 mL

 

 

Fi

Figure 1. HeLa cells were incubated in 1X HBSS buffer with 5% serum to induce starvation. Following starvation, cells were treated with Autophagy Green™ (Cat No. 23002) working solution for 20 minutes in a 37°C, 5% CO2 incubator and then washed 3 times. Nuclei were labeled with Hoechst 33342 (Cat No. 17530). Lysosomes were labeled with LysoBrite™ Orange (Cat No. 22657).

 

Figure 2. Image of HeLa cells stained with Cell Navigator™ Lysosomal Staining Kit in a Costar black wall/clear bottom 96-well plate using an Olympus fluorescence microscope TRITC channel.

LysoBrite™ Deep Red Overview

Catalog Number:
22646
Unit Size:
500 Tests
Molecular weight Excitation (nm) Emission (nm)
746.99 595 618

 

Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains this pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels. LysoBrite™ Deep Red selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. LysoBrite™ dyes significantly outperform the equivalent LysoTracker ™dyes (from Invitrogen). LysoBrite™ dyes can stay in live cells for more than a week with very minimal cell toxicity while the LysoTracker dyes can only be used for a few hours. LysoBrite™ dyes can survive a few generations of cell division. In addition, LysoBrite™ dyes are much more photostable than the LysoTracker dyes.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of LysoBrite™ Deep Red to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg 0.5 mg 1 mg 5 mg 10 mg
1 mM 133.871 µL 669.353 µL 1.339 mL 6.694 mL 13.387 mL
5 mM 26.774 µL 133.871 µL 267.741 µL 1.339 mL 2.677 mL
10 mM 13.387 µL 66.935 µL 133.871 µL 669.353 µL 1.339 mL

 

Figure 1. Image of Hela cells stained with the A: LysoBrite™ Deep Redor B: LysoTracker® Red DND-99 (from Invitrogen) in a Costar black 96-well plate. The TRTIC signals were compared at 0 and 120 seconds exposure time by using an Olympus fluorescence microscope.

 

Figure 2. Image of Hela cells stained with LysoBrite™ Deep Red.

LysoBrite™ Red DND-99 Overview

Catalog Number:
22647
Unit Size:
500 Tests
Molecular weight Excitation (nm) Emission (nm)
399.25 573 592

 

LysoBrite Red DND-99 is chemically same to the LysoTracker® Red DND-99 used for labeling and tracking acidic organelles in live cells (LysoTracker® is the trademark of ThermoFisher). It has good selectivity for acidic organelles, and reasonably retaining its staining pattern after aldehyde fixation. The LysoBrite™ probes consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH, allowing them to freely permeate cell membranes to label live cells.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of LysoBrite™ Red DND-99 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg 0.5 mg 1 mg 5 mg 10 mg
1 mM 250.47 µL 1.252 mL 2.505 mL 12.523 mL 25.047 mL
5 mM 50.094 µL 250.47 µL 500.939 µL 2.505 mL 5.009 mL
10 mM 25.047 µL 125.235 µL 250.47 µL 1.252 mL 2.505 mL

 

 

 

Figure 1. Chemical structure for LysoBrite™ Red DND-99.

For more information please email info@scienze.nz  and one of our technical specialists will contact you directly to discuss your needs.

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